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Cantharidin inhibits osteosarcoma cell migration and invasion in vitro measured with Western blot assay and xCELLigence RTCA DP system. 143B and U-2 OS cells were respectively exposed to 0, 1.25, 2.5 or 5μM cantharidin: (A) 143B cell migration; (B) U-2 OS cell migration; (C) 143B cell invasion; (D) U-2 OS cell invasion; (E) Western blot detected expression of EMT-related factor Vimentin with β-actin as a loading control. *p < 0.05, **p < 0.01, ***p < 0.001.
