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. 2021 Jun 22;17(10):2633–2651. doi: 10.7150/ijbs.58514

Figure 3.

Figure 3

YTHDC2 downregulation promotes lung cancer cell proliferation. Representative images and quantification results of cell cycle of (A) BEAS-2B and (C) H1299 cells transfected with siYTHDC2 and NC. Representative images and quantification results of cell cycle of (B) S30 and (D) H1299 cells transfected with overexpression vector (pYTHDC2) and blank vector. (E) Cell proliferation was measured in BEAS-2B and H1299 cells transfected with siYTHDC2 and NC by EdU cell proliferation assay. (F) Cell proliferation were evaluated in S30 and H1299 cells transfected with pYTHDC2 and blank control by EdU cell proliferation assay. (G) Representative immunofluorescence images and (I) quantification results of Ki67 in BEAS-2B and H1299 cells transfected with siYTHDC2 and NC. (H) Representative immunofluorescence images and (J) quantification results of Ki67 in S30 and H1299 cells transfected with pYTHDC2 and blank control. Relative mRNA expression level of cyclin D1 in (K) BEAS-2B and H1299 cells transfected with siYTHDC2 and NC, as well as in (L) S30 and H1299 cells transfected with pYTHDC2 and blank control. *P<0.05 vs. NC or blank control group, **P<0.01 vs. NC or blank control group. YTHDC2, YTH domain containing 2; siRNA, small interfering RNA; NC, negative control; EdU, 5-ethynyl-2'-deoxyuridine.