Figure 2.

GSDMB regulates the glycolysis of bladder cancer cells. A-B. T24 cell lines were infected with constructed siGSDMB. After infecting 72h, cells were harvested for RNA-Seq. Heatmap (A) and volcano plot (B) were used to show the differential expressed genes. C-D. GO enrichment analysis (C) and KEGG enrichment analysis (D) based on differential genes through RNA-Seq. There were significant differences in glycolysis changes. E-H. T24 cell lines were infected with constructed plasmid. After infecting 48h and 72h, all cells were harvested for RT-qPCR (F) and Western Blotting analysis (E). Glucose consumption (original level minus remaining amount) (G) and lactate production (H) were measured in the spent medium of T24 cells. All data were presented as Means ± SD (n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001. I-L. T24 cell lines were infected with constructed plasmid. After infecting 48h and 72h, all cells were harvested for RT-qPCR (J) and Western Blotting analysis (I). Glucose consumption (original level minus remaining amount) (K) and lactate production (L) were measured in the spent medium of T24 cells. All data were presented as Means ± SD (n = 3). **, P < 0.01; ***, P < 0.001.