Figure 5.

USP24 interacts with GSDMB to up-regulate the GSDMB protein level in bladder cancer. A. Western blot analysis of co-immunoprecipitated protein samples from T24 and 5637 cells lines. B. GEPIA network tool analyzed the RNA correlation between GSDMB and USP24. C. The typical IHC staining images using TMA tissue chip (n = 80). D. Heatmap and scatter diagram were used to reveal the correlation of protein expression between GSDMB and USP24 in bladder cancer patient specimens. (n=80, spearman correlation r = 0.6583, P < 0.001). E-H. T24 and 5637 cell lines were infected with constructed plasmids (shUSP24#1, shUSP24#2, HA-USP24). After infecting 48h and 72h, all cells were harvested for RT-qPCR (F and H) and Western Blotting analysis (E and G). All data were showed as Means ± SD (n = 3). ns, no significant. I-J. T24 and 5637 cell lines were treated with EOAI3402143 for 48h and 72h. Then cells were harvested for RT-qPCR (I) and Western Blotting analysis (J). All data were showed as Means ± SD (n = 3). ns, no significant.