Figure 2.

Depletion of KDM4B alters the extracellular acidification rate (EACR) and oxygen consumption rate (OCR). The ECAR (A) and OCR (C) of KDM4B-knockdown (sh4B#1 or sh4B#2) or control (LKO) C4-2B were measured by the Seahorse analyzer. For the ECAR assay, adherent cells were incubated at glucose-free and NaHCO₃-free medium, followed by injection of 10 mM glucose, 1 µM oligomycin (Oligo), and 50 mM 2-DG. For the OCR assay, adherent cells were incubated at NaHCO₃-free medium with subsequent injection of 1 μM oligomycin (Oligo), 0.5 μM FCCP, and 50 μM rotenone/antimycin A (RA). (B) The glycolytic capacity was plotted based on the ECAR assay (A). (D-F) are metabolic parameters inferred from the OCR assay (C), basal respiration (D), ATP production (E), and maximal respiration (F). All data are presented as the mean ± S.D. and p values were calculated by two-sided Student's t-test.