Figure 7.
ENO1 interacts with AKT1. (A) Co-IP detected the interaction of exogenous ENO1 and AKT1. (B) Colocalization of ENO1 and AKT1 in HEK293T cells was detected by immunofluorescence staining. (C) Co-IP detected the interaction of endogenous ENO1 and CCDC65 with AKT1. (D) Schematic representations of AKT1 and its mutants. The full-length of AKT1 is important for interaction with ENO1. The mutants of AKT1 were transfected into HEK293T cells and analyzed by immunoprecipitation using anti-Myc antibody. (E) Co-IP detected the effects of si-CCDC65 on the interaction of ENO1 and AKT1 in GC cells.