Skip to main content
. 2021 Jul 6;11(16):7995–8007. doi: 10.7150/thno.50990

Figure 2.

Figure 2

Deletion of Miat attenuates Ang II-induced cardiac pathological hypertrophy. At 28 days after continuous Ang II (2 mg/Kg BW/day) or saline treatment, mice were euthanized and examined. (A) Heart weight (HW) versus tibia length (TL) ratio. (B) Representative images of wheat germ agglutinin (WGA) staining (left panel) and quantification of cardiomyocyte cross-section areas (right panel). (C) Representative images of TUNEL and cTnI staining (left panel) and quantification of apoptotic cardiomyocytes (right panel, % of nuclei). Yellow arrows indicate TUNEL+ cardiomyocytes. Scale bar = 50µm. (D) Representative images of Sirius Red/Fast Green staining (left panel) and quantification of interstitial fibrosis areas (right panel). (E) Representative images of CD31 staining (left panel) and quantification of capillary density (right panel). HPF, high-powered field. (F) qRT-PCR analyses of the mRNA levels of Bnp and Anf in the heart tissue. *p < 0.05, **p < 0.01.