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Regulation of PCa cell death upon glutamine deprivation. (A) Analysis of cell viability using Trypan blue exclusion assay. Data are mean ± s.d. (B) Western blot analysis of PARP cleavage after glutamine starvation of PCa cells for 24 h. Cells grown in the presence of glutamine were used as control. Data are mean ± s.e.m. (C) Cell death has been evaluated by flow cytometry using Annexin V-FITC and PI staining. Data are mean ± s.d. *p < 0.05; **p < 0.01.
