Table 2.
Summary of the 19 unique clones identified.
| Number | mAbs ID | Competition FACS | Clones selected | Group | Heavy chain | Light chain | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Germline | CDR3 lengths | %SHM | Germline | CDR3 lengths | %SHM | |||||
| 1 | 2H1 | ★★ | 1 | IGHV3-9*01 | 19 | 2.7 | IGLV2-14*01 | 10 | 0.7 | |
| 2 | 1A10 | ★★★ | 1 | IGHV3-9*01 | 19 | 2.7 | IGLV2-14*01 | 10 | 0.7 | |
| 3 | 3A9 | ★★★ | ▲ | 1 | IGHV3-9*01 | 19 | 2.4 | IGLV2-14*01 | 10 | 1.4 |
| 4 | 1F2 | ★★★★ | ▲ | 2 | IGHV3-66*01 | 12 | 1.4 | IGKV3-20*01 | 9 | 0.7 |
| 5 | 1D10 | ★★★★ | ▲ | 3 | IGHV3-53*04 | 12 | 2 | IGKV1-12*01 | 8 | 0.7 |
| 6 | 1H5 | ★★★ | 3 | IGHV3-53*04 | 12 | 2.1 | IGKV1-9*01 | 9 | 1.8 | |
| 7 | 1B6 | ★★★★ | ▲ | 4 | IGHV3-66*01 | 12 | 2.7 | IGLV3-1*01 | 9 | 6.1 |
| 8 | 1B9 | ★★ | 4 | IGHV3-53*02 | 12 | 2.4 | IGKV1-27*01 | 9 | 2.8 | |
| 9 | 1H8 | ★★★★ | ▲ | 4 | IGHV3-66*01 | 12 | 2.7 | IGKV1-27*01 | 9 | 2.1 |
| 10 | 1D6 | ★★★★ | 4 | IGHV3-53*02 | 12 | 2.4 | IGLV3-21*03 | 11 | 0 | |
| 11 | 2B11 | ★★★★ | ▲ | 4 | IGHV3-66*01 | 12 | 2.7 | IGLV1-44*01 | 12 | 8.4 |
| 12 | 2B8 | ★★★ | 4 | IGHV3-66*01 | 12 | 2.4 | IGLV1-44*01 | 12 | 11.5 | |
| 13 | 1E7 | ★★★★ | 5 | IGHV3-53*01 | 11 | 0.7 | IGKV1-12*01 | 10 | 0.4 | |
| 14 | 1D7 | ★★★★ | ▲ | 5 | IGHV3-53*01 | 11 | 1 | IGKV1-39*01 | 11 | 0.4 |
| 15 | 2A2 | ★★★★ | ▲ | 5 | IGHV3-53*01 | 11 | 1.4 | IGLV2-14*01 | 10 | 0.7 |
| 16 | 1E10 | ★★★ | ▲ | 6 | IGHV1-69*04 | 19 | 0.3 | IGKV1-39*01 | 9 | 0.4 |
| 17 | 2H8 | ★ | 7 | IGHV3-30*18 | 19 | 1.4 | IGLV3-21*02 | 11 | 1.8 | |
| 18 | 2H2 | ★ | 8 | IGHV1-69*10 | 17 | 0.7 | IGLV1-40*01 | 11 | 2.3 | |
| 19 | 1B10 | ★★★★ | ▲ | 9 | IGHV3-53*02 | 11 | 1 | IGLV2-8*01 | 10 | 2.7 |
IGHV the immunoglobulin heavy-chain gene-variable region, IGLV the immunoglobulin lambda light-chain gene-variable region, IGKV the immunoglobulin kappa light-chain gene-variable region.
Five ★ represent for clones with strong competition. The triangle-marked (▲) clones were chosen for IgG conversion, based on their competition and diversity.