TABLE 3.
Osmolality measurements of increasing NADES concentration (%wt/vol) in normosol-R and MCOa
| CPA solution | Osmolality (mOsm/kg) |
|---|---|
| 5% T:G | 640 ± 10 |
| 10% T:G | 1,350 ± 60 |
| 15% T:G | 1710 ± 10b |
| TGI (MCO) | 1,340 ± 10 |
a
NADES: natural deep eutectic system; MCO or TGI: a multicomponent osmolyte solution containing trehalose, glycerol and isoleucine; CPA: cryoprotective agent; T:G: NADES composed of trehalose and glycerol at 1:30 M ratio. 95% confidence interval calculated from 3 independent replicates for each sample using a T distribution.
b
Estimated osmolality extrapolated using a dilution curve (Figure S1).