TABLE 4.
Post-thaw recovery of Jurkat cells cryopreserved in NADES, MCO, and DMSO solutionsa
| CPA solutions | Post-thaw recovery (%)n.s. | CPA solutions | Post-thaw recovery (%)n.s. |
|---|---|---|---|
| 5% T:G | 75.8 ± 6.3 | 5% T:G + 10.75 mM isoleucine | 86.1 ± 16.7 |
| 10% T:G | 77.2 ± 5.5 | 5% T:G +21.5 mM isoleucine | 76.0 ± 13.0 |
| 15% T:G | 81.9 ± 6.4 | 5% T:G + 43 mM isoleucine | 78.7 ± 15.8 |
| TGI (MCO) | 79.8 ± 5.7 | 10% DMSO | 82.2 ± 13.9 |
Abbreviations: CPA, cryoprotective agents; DMSO, dimethyl sulfoxide; n. s., not significant; NADES, natural deep eutectic system; T:G, NADES composed of trehalose and glycerol at 1:30 M ratio; TGI or MCO, a multicomponent osmolyte solution containing trehalose, glycerol, and isoleucine.
a
95% confidence interval calculated from six biological replicates for each sample using a T distribution. p > .05 in ANOVA with Bonferroni correction for all samples.