TABLE 5.
Post-thaw apoptosis of Jurkat cells cryopreserved in NADES, MCO and DMSO solutionsa
| CPA solutions | Live (%) | Early apoptotic (%) | Late apoptotic (%) | Necrotic (%) | |
|---|---|---|---|---|---|
| NADES solutions | 5% T:G | 89.97 ± 0.29 | 9.03 ± 0.39 | 0.34 ± 0.17 | 0.65 ± 0.48 |
| 10% T:G | 52.37 ± 2.01b | 46.40 ± 2.15b | 0.58 ± 0.15b | 0.68 ± 0.73 | |
| 15% T:G | 47.07 ± 7.52b | 50.83 ± 8.61b | 1.29 ± 0.16b | 0.80 ± 0.89 | |
| 5% T:G + 10.75 mM isoleucine | 94.97 ± 0.52b | 4.45 ± 0.33b | 0.22 ± 0.087 | 0.37 ± 0.18 | |
| 5% T:G + 21.5 mM isoleucine | 88.70 ± 0.25b | 10.43 ± 0.14b | 0.42 ± 0.20 | 0.42 ± 0.11 | |
| 5% T:G + 43 mM isoleucine | 80.80 ± 0.90b | 18.03 ± 0.76b | 0.62 ± 0.38b | 0.50 ± 0.13 | |
| TGI (MCO) | 90.30 ± 1.14b | 8.91 ± 1.29b | 0.36 ± 0.029 | 0.42 ± 0.19 | |
| 10% DMSO | 92.87 ± 0.14b | 5.90 ± 3.19b | 0.27 ± 0.12 | 0.32 ± 0.28 |
Abbreviations: CPA, cryoprotective agents; DMSO, dimethyl sulfoxide; NADES, natural deep eutectic system; T:G, NADES composed of trehalose and glycerol at 1:30 M ratio; TGI or MCO, a multicomponent osmolyte solution containing trehalose, glycerol, and isoleucine.
a
95% confidence interval calculated from three independent replicates for each sample using a T distribution. Fresh Jurkat cells in culture were 99.1% live, 0.29% early apoptotic, 0.03% late apoptotic, and 0.56% necrotic.
b
p < .05 in two-sample comparison with 5% T:G.