Table 2. Expression Levels of 6xHis-Twin-Strep-Tagged SARS-CoV-2 Proteins in E. coli LOBSTR-RIL.

protein	expression levela
NSP1	soluble: L, 16; M, 37
	insoluble: N, 16; M, 37
NSP2	soluble: M, 16; L, 37
	insoluble: N, 16; H, 37
NSP3	soluble: L, 16; L, 37
	insoluble: N, 16; N, 37
NSP3 ARH	soluble: M, 16; L, 37
	insoluble: N, 16; H, 37
NSP3 PLpro	soluble: L, 16; N, 37
	insoluble: L, 16; H, 37
NSP3C	soluble: H, 16; L, 37
	insoluble: L, 16; H, 37
NSP4N	soluble: L, 16; N, 37
	insoluble: M, 16; H, 37
NSP4C	soluble: L, 16; L, 37
	insoluble: N, 16; H, 37
NSP5	soluble: N, 16; M, 37
	insoluble: N, 16; L, 37
NSP7	soluble: L, 16; H, 37
	insoluble: N, 16; L, 37
NSP8	soluble: M, 16; H, 37
	insoluble: L, 16; L, 37
NSP9	soluble: L, 16; H, 37
	insoluble: N, 16; L, 37
NSP10	soluble: M, 16; L, 37
	insoluble: N, 16; H, 37
NSP12	soluble: L, 16; L, 37
	insoluble: H, 16; H, 37
NSP12P	soluble: L, 16; L, 37
	insoluble: H, 16; H, 37
NSP13	soluble: M, 16; N, 37
	insoluble: H, 16; H, 37
NSP14	soluble: L, 16; N, 37
	insoluble: L, 16; H, 37
NSP15	soluble: L; 16; L, 37
	insoluble: N, 16; L, 37
NSP16	soluble: M, 16; M, 37
	insoluble: L, 16; H, 37
S	soluble: L, 16; N, 37
	insoluble: H, 16; H, 37
S1	soluble: L, 16; N, 37
	insoluble: H, 16; H, 37
S2	soluble: L, 16; N, 37
	insoluble: M, 16; H, 37
ORF3a	soluble: M, 16; L, 37
	insoluble: L, 16; H, 37
M	soluble: L, 16; N, 37
	insoluble: L, 16; H, 37
ORF7a	soluble: L, 16; L, 37
	insoluble: L, 16; H, 37
ORF8	soluble: L, 16; N, 37
	insoluble: M, 16; H, 37
ORF9b	soluble: L, 16; M, 37
	insoluble: N, 16; M, 37
N	soluble: L, 16; L, 37
	insoluble: M, 16; H, 37
NS	soluble: M, 16; H, 37
	insoluble: N, 16; M, 37

^aL, low; M, moderate; H, high; N, none; 16 for 16 °C; and 37 for 37 °C. For example, L, 16 indicates low expression at 16 °C.