Table 3.
NGS systems and HLA Loci typed by participating laboratories.
| Labcode | Software | Hardware | HLA loci typed | Coverage class I/class II |
|---|---|---|---|---|
| areelg* | NGSengine, GenDx | MiSeq, Illumina | A, C, B, DRB1/3/4/5, DQA1, DQB1, DPA1, DPBI | Full-gene/wide-coverage |
| ausdes | NGSengine, GenDx | Ion S5™ system | A, C, B, DRB1/3/4/5, DQB1, DPB1 | Full-gene/wide-coverage |
| gbrcn | TypeStream™ Visual, One lambda Thermo Fisher Scientific | Ion S5™ system | A, C, B, DRB1/3/4/5, DQA1, DQB1, DPA1, DPBI | Full-gene/full-gene (DQA1, DPA1) and wide-coverage |
| gbrpep* | NGSengine, GenDx | MiSeq, Illumina | A, C, B, DRB1/3/4/5, DQA1, DQB1, DPA1, DPBI | Full-gene/wide-coverage |
| grcsta | HLA Twin, Omixon | MiSeq, Illumina | A, C, B, DRB1, DQA1, DQB1, DPB1 | Full-gene/wide-coverage |
| indkan | NGSengine, GenDx | MiSeq, Illumina | A, C, B, DRB1, DQA1, DQB1, DPA1, DPB1 | Full-gene/wide-coverage |
| indnar | MIA FORA FLEX, Immucor | MiniSeq, Illumina | A, C, B, DRB1, DQB1 | Full-gene/wide-coverage |
| itasac | NGSengine, GenDx | MiSeq, Illumina | A, C, B, DRB1, DQB1 | Full-gene/wide-coverage |
| mexgor‡ | MIA FORA FLEX, Immucor | MiSeq, Illumina | A, C, B, DRB1/3/4/5, DQA1, DQB1, DPA1, DPB1 | Full-gene/wide-coverage |
| ucamor | TruSight HLA Assign, Illumina | MiSeq, Illumina | A, C, B, DRB1/3/4/5, DQA1, DQB1, DPA1, DPBI | Full-gene (A, C), B (exon1-intron 6) /full-gene (DQ DPA1) and wide-coverage |
| uilmar | NGSengine, GenDx | MiSeq, Illumina | A, C, B, DRB1/3/4/5, DQA1, DQB1, DPA1, DPBI | Full-gene/wide-coverage |
| umdtho | NGSengine, GenDx | MiSeq, Illumina | A, C, B, DRB1, DQB1, DPB1 | Full-gene/wide-coverage |
| ussta1 | MIA FORA FLEX, Immucor | MiniSeq and NextSeq, Illumina | A, C, B, DRB1/3/4/5, DQA1, DQB1, DPA1, DPB1 | Full-gene/wide-coverage |
*
Samples typed by laboratory utxask (Baylor University Medical center).
‡
Samples typed by laboratory ussta1 .