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Effects of deletion of CcpA on mannitol production
| Conditiona | Strainb | Mannitol produced (g/liter) | Yield (%) | Biomass (OD600) |
|---|---|---|---|---|
| TSF | AceF | 6.08 | 0.6 | |
| AceCF | 5.92 | 0.59 | ||
| AceR-mtlD | 10.1 | 0.55 | ||
| AG | AceF | 2.18 | 0.41 | 1.38 |
| AceCF | 0.62 | 0.34 | 0.65 | |
| AceR-mtlD↑ | 0.71 | 0.34 | 0.47 |
TSF, two-step fermentation; AG, anaerobic growth. For anaerobic growth, 35 mM arginine was added, and biomass and yield were measured and calculated after 7 days of incubation.
In strain AceR-mtlD, mtlD is overexpressed.
