TABLE 3.
Primers for RT-qPCR analysis and strain construction used in this study
| Purpose or pathway | Gene/primer | Primer sequence(s) (5′ to 3′) |
|---|---|---|
| RT-qPCR | ACT1 | ATTATATGTTTAGAGGTTGCTGCTTTGG and AATTCGTTGTAGAAGGTATGATGCC |
| ZNF1 | AGGCACTAATTGATCAGTGTCTGC and GCAGAAACTGGATAACTGTATCC | |
| UPR pathway | HAC1 | ACTGAACAGCGTCAACCTTG and GTAGCGTCGTCGACTCTG |
| HSP30 | GCAAGTCTATCACAGGTG and AACATAGCGACAGCACCA | |
| HSP104 | TGCACCTGCGGAGATAAC and GCGGTCTTACCGATACCTG | |
| IRE1 | GATATACTAATCGCAGCCGACG and GTAACCCTTGATGGGCGT | |
| KAR2 | CGTGGCATTCACCGATGA and GATGGTACCAGCATCCTTGG | |
| LHS1 | CTATATTGCAGCCGGAGGAC and GCCTCAGAATTGGCGCTTA | |
| SSA1 | AGGCTGACATGAAGCACTTC and AGCATCCTTGGTAGCTTGTC | |
| Glycerol metabolism | DAK1 | TATTAACTGCCGCGTTGC and TTAGCTGTACCGTCTAAGCC |
| GAT1 | TGCATAACCGCACGTCTT and GAGCCAGGCTTCACTATAGATG | |
| GPD1 | GTGGGTGTTCGAAGAAGAG and ACAGGAGATAGCTCTGACG | |
| GPP1 | GAAGTTCCAGGTGCTGTC and CGTTGTATTCACCGACTC | |
| GPP2 | CCGGTCAAGTACGGTGAA and CAATACCTGCTGGAGCGT | |
| GUP1 | GAACGTCTCCTATCTCAAGG and TGCTGATGGTGGCTATTC | |
| GUT1 | CTGCAGAAGGCTATGCCA and CCTTGTAAGGTGGGAGACC | |
| GUT2 | TCGACGTGTTGATCATCGG and AATTGGTAGCACCGTGCA | |
| Fatty acid biosynthesis | FAS1 | CTAGTCGAACCTTCCAAGGTC and GTGTTACCTTGACCACCGA |
| OLE1 | CATCTCCGAACAACCATGGA and ATCTCAATGGCCAGTGAGC | |
| Carbohydrate metabolism | ATH1 | ACATACTCACATGCCTCGAG and CTAAGGCATAGCCGAACC |
| GPH1 | GCTTATGAAGCTGCTTCG and CGGTTCTTGGTCCAAGACA | |
| GSY1 | CGAATGGAAGGCTGACCTA and ATGCCTCTCTTACCAGCTTC | |
| NTH1 | ACAGACTAGACGTGGTTCTG and CGGTATCCTCGATGGTCA | |
| PGM2 | AGATTGCCGCTATCGGTG and TTGTGACGGATTCAGGAGC | |
| TSL1 | GCGAATGCAACTACCTCACA and GTTGCTGTTGGATTGGCG | |
| UGP1 | CAGTACGATAGCGACGTGC and GTGACCTGGTGGATACCAAG | |
| Cell wall biosynthesis | FKS1 | ATATGGCTGCTCAAGACGG and GAGGACCTAGAGTCCAAGAGAA |
| SED1 | CGGTACTTCTACTGAAGCTCC and CAGTGACGGTGTAAGTCTTACC | |
| SMI1 | ATCACTCACGCAGAGGAAGA and GTGCATATACCGGTTGCACT | |
| Strain construction | D1306 | CAAGAACTTCGTATCGGCTTTC |
| D1307 | GCTAGAGACATGTTAGAAGAC | |
| D1308 | CATTAGCAGAGTCGGTAGCTA | |
| D1309 | TTCTAGAACTAGTGGATCCATGGCCCGCAATAGACAAGCG | |
| D1310 | AACTAATTACATGACTCGAGTTAAGGAAGCGCATCTACATC | |
| D1321 | GATCATCATAACACAGCAAAGGC | |
| C3586 and C3587 | CAAAGGTTGATGCAAGTCGA and GTAATCCCTTCCACCTTTCT | |
| C4330 and C4331 | GGAGCAGACATCACTAAACG and GCCACAACCAAGTGAGATAC |