FIG 6.

Selection for Gad island and for gadE⁺. The slopes were calculated over days 0 to 3 of testing. Significance was determined as for Fig. 3 (†, P < 0.05). (A) slp-gadX⁺. Selection rate is given by log₂(W3110/Δslp-gadX)/day. Conditions include LBK-PIPES pH 6.8 alone (n = 12), with 0.15% bile acids (n = 20), with 6 mM salicylate (n = 12), and with 0.15% bile acids and 6 mM salicylate (n = 12). (B) gadE⁺. Selection rate is given by log₂(ΔyhdN::kanR/ΔgadE::kanR)/day. Conditions include LBK-PIPES pH 6.8 alone (n = 12), with 0.15% bile acids (n = 20), with 6 mM salicylate (n = 12), and with 0.15% bile acids and 6 mM salicylate (n = 16).