Figure 5.

Vaccination with polymersome-formulated RBD improves antigen-specific T cell responses in mice. Cells isolated from dLN of PS-vaccinated mice 1 week postboost were restimulated ex vivo for 6 h with RBD peptide pools or 3 days with full RBD protein and analyzed by flow cytometry or multiplexed ELISA, respectively. (a,b) Percentages of cytokine-positive CD8⁺ T cells (a) and CD4⁺ T cells (b) in response to RBD peptide pools, subtracting unstimulated controls. (c) Proinflammatory cytokine levels in the supernatant measured after restimulation with whole RBD protein. Data plotted as mean ± SD and represent 1 of 2 experiments with n = 5 mice each. Symbols represent individual mice. Comparisons to unadjuvanted RBD_free were made using one-way ANOVA with Dunn’s post-test.