Figure 3.
Early-onset changes in CFA layer 5B membrane potential dynamics
(A) Patch-clamp recording in layer5B CFA. IN, interneuron; MThDN/IPN, dentate/interpositus nucleus-recipient region of motor thalamus.
(B) Single-trial subthreshold membrane potential (Vm) trajectories from two layer 5B projection neurons (spikes clipped). MI, movement initiation.
(C) Peri-movement ΔVm ± 95% CI. Purple and white indicate significant and non-significant changes in ΔVm, respectively (n = 23 neurons from N = 23 mice).
(D) Overlaid peak scaled subthreshold Vm split by direction of change (left: depolarizing, n = 15/23 neurons; right: hyperpolarizing, n = 4/23 neurons). Thick line, population mean ± 95% CI; green dotted line, mean MThDN/IPN activity onset ± 95% CI (green shading) shown in Figure 2E; black dashed line, movement initiation (MI).
(E) Cumulative probability of ΔVm onsets (n = 19/23 neurons).
(F) Movement-related subthreshold ΔVm and firing rate change correlation. Symbols, mean ± 95% CI from individual neurons; black line, linear fit to the data (Pearson’s r).