Figure 4.
Activity in DN/IPN and MThDN/IPN is required for cue-triggered movement initiation
(A) Muscimol inactivation of MThDN/IPN or DN/IPN.
(B) Left: modal spread of fluorescent muscimol 10 min after injection into DN/IPN (inset, location of DN/IPN). Black cross, median point of IPN injection (N = 4 mice). Right: number of successful push trials 10 min after injection of saline (black, N = 6 mice) or muscimol (green, N = 6 mice ), two-sample t test. Symbols, population means ± 95% CI. FN, fastigial nucleus; IPN, interpositus nucleus; DN, dentate nucleus.
(C) Left: modal spread of fluorescent muscimol 10 min after injection into MThDN/IPN (inset, location of thalamic nuclei). Black cross, median point of injection (N = 4 mice). Right: number of successful push trials 10 min after injection of saline (black, N = 5 mice) or muscimol (green, N = 5 mice), two-sample t test. Symbols, population means ± 95% CI. AM, anteromedial; VL, ventrolateral; VPM, ventral posteromedial; VPL, ventral posterolateral; VM, ventromedial nuclei.
(D) Patch-clamp recording in layer 5B CFA during muscimol inactivation of MThDN/IPN. I-Clamp, current clamp.
(E) Subthreshold ΔVm ± 95% CI from a layer 5B projection neuron before (Pre; black) and after muscimol injection (Post; green) targeted to MThDN/IPN.
(F) Ratio of normalized area under the curve for Vm trajectories before (Pre) and after (Post) muscimol injection into MThDN/IPN. Data grouped by Vm change prior to muscimol injection. Green symbols, population means ± 95% CI (n = 10 cells from N = 10 mice).
(G) Left: change in firing rate before (Pre) and after (Post) muscimol injection into MThDN/IPN. Colored symbols, population means ± 95% CI; dotted lines, individual neurons (n = 8 cells from N = 8 mice); black lines, neurons with no change in firing rate prior to muscimol injection (n = 2 cells from N = 2 mice). Right: number of successful push trials 10 mins after injection of muscimol (green, N = 10 mice).