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. 2021 Jul 13;12:693054. doi: 10.3389/fimmu.2021.693054

Figure 1.

Figure 1

In vitro priming of SARS-CoV-2-specific naive CD8+ T cells in healthy donors. (A) Scheme of the in vitro priming protocol. (B) PBMCs (5 × 106) were primed with EV10 or a pool of 37 SARS-CoV-2-derived peptides. After ten days, the frequency of peptide-specific primed naive CD8+ T cells was measured by IFNγ ELISpot (left), and the expression of IFNγ, TNF, and CD107 (right) was assessed by ICS. Data are shown, after background (NT) subtraction, as the mean + S.E.M. of two donors. (C, D) PBMCs (5–100 × 106) were primed with a pool of 37 SARS-CoV-2-derived peptides. After ten days, the frequency of epitope-specific primed naive CD8+ T cells was measured by IFNγ ELISpot, and the expression of IFNγ, TNF, and CD107 was assessed by ICS. Data are shown, after background (NT) subtraction, as the mean + S.E.M. of three donors (C). One representative dot plot for stimulation of 5 and 70 × 106 PBMCs is shown (D).