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. 2021 Jul 23;14:3261–3276. doi: 10.2147/JIR.S316314

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© 2021 Li et al.

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Immunofluorescence double staining of inflammatory marker CD16 and anti-inflammatory marker CD206 on microglia in the substantia nigra of mice. (A) Staining of Iba-1 (green) and CD16 (classical microglia marker, red) in the SNpc and (B) quantification of the percentage of CD16⁺/Iba‐1⁺ cell. (C) Double staining of Iba-1 (microglia marker, green) and CD206 (alternative microglia marker, red) in the SNpc for immunofluorescence pictures and (D) quantification of the percentage of CD206⁺/Iba‐1⁺ cell. Scale bar is 20 µm. Data represent the means ± SEM; Statistics one-way ANOVA; ^##P < 0.01, ^###P < 0.001 vs Control group; *P < 0.05, vs MPTP group; n = 3.