Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Xenopus laevis) |
Tadpole | Nasco | WT and Albino strains; LM00456, LM00456(A) | Bred in house |
| Chemical compound, drug | Valproic Acid | Sigma | Cat. No. P4543 | Drug |
| Chemical compound, drug | SB-3CT | Tocris | Cat. No. 6088 | MMP9/2 inhibitor |
| Recombinant DNA reagent | pCMV-SPORT6, MMP-9, GFP | Invitrogen | pCMV-SPORT6 (backbone) | Expressing Xenopus MMP9 and GFP, or GFP alone, or MMP9-FLAG |
| Genetic reagent | MMP-9 MO | GeneTools Bestman and Cline, 2014 | DOI:10.1007/978-1-62703-655-9_11 | Morpholino antisense oligonucleotide- lissamine tagged, Used to drive down expression of MMP9 |
| Genetic reagent | Control MO | GeneTools Bestman and Cline, 2014 | DOI:10.1007/978-1-62703-655-9_11 | Used as a transfection control, Morpholino antisense oligonucleotide- lissamine tagged |
| Antibody | Anti-MMP9 (Rabbit polyclonal) | EMD Millipore | Ab19016; RRID:AB_91090 | WB (1:2000) |
| Antibody | Anti-Rabbit-HRP (goat polyclonal) | BioRad | #1662408EDU; RRID:AB_11125345 | Secondary Ab for WB |
| Commercial assay or kit | Pierce ECL-western blot substrate | Thermo Fisher | Cat#32209 | For visualizing labeling |
| Recombinant DNA reagent | pCALNL-GFP (plasmid) | Schohl et al., 2020 , DOI: 10.3389/fncir.2020.00047 | For single cell labeling | |
| Recombinant DNA reagent | pCAG-Cre (plasmid) | Schohl et al., 2020, DOI: 10.3389/fncir.2020.00047 | For single cell labeling | |
| Recombinant DNA reagent | pBE-PSD-95 GFP | Sanchez et al., 2006; DOI: 10.1242/dev.02409 | For synaptic labeling | |
| Recombinant DNA reagent | pCMV-mCherry | Schwartz et al., 2009; DOI:10.1016/j.neuron.2011.02.055 | Cell filling mCherry | |
| Chemical compound, drug | F-WFA | Vector Labs | FL-1351–2 | Fluorescein-labeled Wisteria floribunda agglutinin, 1:500 dilution for staining PNNs |