| cell line (Homo-sapiens) |
SEM |
DSMZ |
ACC546 |
|
| cell line (Homo-sapiens) |
MOLM13 |
Duo laboratory University of Michigan |
|
Verified by STR profiling ATCC |
| cell line (Homo-sapiens) |
THP-1 |
ATCC |
TIB-202 |
Verified by STR profiling ATCC |
| cell line (Homo- sapiens) |
MV4;11 |
Duo laboratory University of Michigan |
|
Verified by STR profiling ATCC |
| Gene (Homo- sapiens) |
STAT5A-CA |
This paper |
|
Constitutively active human STAT5A. |
| recombinant DNA reagent |
pTRIPZ-STAT5A-CA (plasmid) |
This paper |
|
Lentiviral construct to transfect and express the gene. |
| recombinant DNA reagent |
pTRIPZ-EZH2 (plasmid) |
This paper |
|
Lentiviral construct to transfect and express the gene. |
| recombinant DNA reagent |
Tet-pLKO-puro |
Addgene |
Cat # 21915 |
|
| recombinant DNA reagent |
PLKO-shFLT3 (plasmid) |
This paper |
|
Lentiviral construct to transfect and knock down gene. |
| recombinant DNA reagent |
PLKO-shscrambled (plasmid) |
This paper |
|
Lentiviral construct to transfect and knock down gene. |
| recombinant DNA reagent |
pTRIPZ-GFP (plasmid) |
This paper |
|
Lentiviral construct to transfect and express the gene. |
| antibody |
anti-MEIS1 (Rabbit polyclonal) |
EMD Millipore |
Cat# ABE2864 |
WB (1:1000) |
| antibody |
anti-H3 (Rabbit polyclonal) |
Active Motif |
Cat#: 61277 |
WB (1:5000) |
| antibody |
anti-H3K79me2 (Rabbit polyclonal) |
Abcam |
Cat# ab3594 |
WB (1:2000) |
| antibody |
anti-H3K27me3 (Rabbit polyclonal) |
Cell Signaling |
Cat# 9733S |
WB (1:1000) |
| antibody |
anti-H3K4me3 (mouse monoclonal) |
Cell Signaling |
Cat# 9751S |
WB (1:1000) |
| antibody |
anti-H4 (Rabbit polyclonal) |
Active Motif |
Cat# 61299 |
WB (1:1000) |
| antibody |
anti-H2B (Rabbit polyclonal) |
Proteintech |
Cat# 2S2899-2 |
WB (1:5000) |
| antibody |
anti-FLT3 (Rabbit polyclonal) |
Cell Signaling |
Cat. #: 33462S |
WB (1:500) |
| antibody |
anti-GAPDH (Rabbit polyclonal) |
Cell Signaling |
Cat# 5174S |
WB (1:5000) |
| antibody |
anti-STAT5A (Rabbit polyclonal) |
Cell Signaling |
Cat# 94205T |
WB (1:1000) |
| antibody |
anti-p- STAT5 (Rabbit polyclonal) |
Cell Signaling |
Cat# 9359S |
WB (1:1000) |
| antibody |
anti-EZH2 (Rabbit polyclonal) |
Cell Signaling |
Cat. #: 5246S |
WB (1:1000) |
| antibody |
anti-LEDGF (Rabbit polyclonal) |
Bethyl |
Cat# A300-848A |
WB (1:2000) |
| antibody |
anti-RBBP5 (Rabbit polyclonal) |
Bethyl |
Cat# A300-109A |
WB (1:1000) |
| antibody |
anti-HNRNPK (Rabbit polyclonal) |
Abcam |
Cat# ab70492 |
WB (1:5000) |
| antibody |
anti-MBD3 (Rabbit polyclonal) |
Bethyl |
Cat. #: A302-529A |
WB (1:1000) |
| antibody |
anti-rabbit (secondary HRP-conjugated) |
Cell Signaling |
Cat# 7074S |
WB (1:10000) |
| antibody |
anti-mouse (secondary HRP-conjugated) |
Bethyl |
Cat# 31432 |
WB (1:10000) |
| chemical compound, drug |
Pinometostat (EPZ5676) |
Cayman Chemical |
Cat# a16175 |
|
| chemical compound, drug |
Tandutinib (MLN518) |
Selleckchem |
Cat. #: S1043 |
|
| chemical compound, drug |
EI1 |
Cayman Chemical |
Cat# 19146–1 |
|
| chemical compound, drug |
MI-503 |
Selleckchem |
Cat. #: S7817 |
|
| chemical compound, drug |
PIM1 inhibitor |
MedChemExpress |
Cat# HY-15604 |
|
| chemical compound, drug |
MM-401 |
Duo laboratory University of Michigan |
|
|
| chemical compound, drug |
Propidium iodide |
Alfa Aesar |
Cat # J66584 |
|
| recombinant DNA reagent |
pCMV-Gag-Pol (plasmid) |
Manicassamy lab |
|
Lentiviral construct to transfect for viral particle assembly. |
| recombinant DNA reagent |
pCMV-vsvg (plasmid) |
Manicassamy lab |
|
Lentiviral construct to transfect for viral particle assembly. |
| recombinant DNA reagent |
pTRIPZ-FLT3-ITD |
This paper |
|
Lentiviral construct to transfect and express the gene. |
| recombinant DNA reagent |
RiboZero Gold |
Illumina |
Cat # MRZ11124C |
Ribosomal rRNA removal from total RNA. |
| commercial assay or kit |
NEBNext Ultra Directional RNA Library prep kit |
NEB |
Cat # E7420S |
cDNA library kit. |
| commercial assay or kit |
Trizol reagent |
Life Technologies |
Cat # 15596018 |
RNA extraction. |
| commercial assay or kit |
Zymo Research RNA Clean and Concentrator |
Zymo Research |
Cat # 11-353B |
|
| commercial assay or kit |
NEBNext Ultra II DNA Library prep kit |
NEB |
Cat # E7645 |
DNA library kit. |
| commercial assay or kit |
PowerUP SYBR Green master mix |
Applied Biosystems |
Cat # A25742 |
qPCR reagent |
| commercial assay or kit |
MMLV HP reverse transcriptase |
Lucigen |
Cat # RT80125K |
|
| commercial assay or kit |
Cell TiterGlo 2 |
Promega |
Cat # G924A |
Cell proliferation assay. |
| software, algorithm |
HISAT2 |
Kim et al., 2015 |
|
|
| software, algorithm |
Cufflinks |
Trapnell et al., 2012 |
|
|
| peptide, recombinant protein |
FITC-conjugated Annexin V |
BD Biosciences |
Cat # 556420 |
Apoptosis detection reagent. |
| sequence-based reagent |
HLD-DRA qPCR F |
This paper |
PCR primers |
CTCAGGAATCATGGGCTATCAA |
| sequence-based reagent |
HLA-DRA qPCR R |
This paper |
PCR primers |
CTCATCACCATCAAAGTCAAACAT |
| sequence-based reagent |
HLA-DRB1 qPCR F |
This paper |
PCR primers |
GTGACACTGATGGTGCTGAG |
| sequence-based reagent |
HLA-DRB1 qPCR R |
This paper |
PCR primers |
GCTCCGTCCCATTGAAGAAA |
| sequence-based reagent |
MEF2C qPCR F |
This paper |
PCR primers |
GTCTGAGGACAAGTACAGGAAAA |
| sequence-based reagent |
MEF2C qPCR R |
This paper |
PCR primers |
GAGACTGGCATCTCGAAGTT |
| sequence-based reagent |
FLT3 qPCR F |
This paper |
PCR primers |
ATCATATCCCATGGTATCAGAATCC |
| sequence-based reagent |
FLT3 qPCR R |
This paper |
PCR primers |
GAAGCAGATACATCCACTTCCA |
| sequence-based reagent |
ARID3B qPCR F |
This paper |
PCR primers |
AGACCATACCAAAGATGCTTCC |
| sequence-based reagent |
ARID3B qPCR R |
This paper |
PCR primers |
ATCATCACTCCAGGCCAAAC |
| sequence-based reagent |
STAT5A qPCR F |
This paper |
PCR primers |
CAGATGCAGGTGCTGTACG |
| sequence-based reagent |
STAT5A qPCR R |
This paper |
PCR primers |
TGTCCAAGTCAATGGCATCC |
| sequence-based reagent |
PIM2 qPCR F |
This paper |
PCR primers |
ATGTTGACCAAGCCTCTACA |
| sequence-based reagent |
PIM2 qPCR R |
This paper |
PCR primers |
TCGATACTCGGCCTCGAA |
| sequence-based reagent |
MEIS1 qPCR F |
This paper |
PCR primers |
AGACGATAGAGAAGGAGGATCAA |
| sequence-based reagent |
MEIS1 qPCR R |
This paper |
PCR primers |
CCGTGTCATCATGATCTCTGTT |
| sequence-based reagent |
HOXA9 qPCR F |
This paper |
PCR primers |
AGGCGCCTTCTCTGAAA |
| sequence-based reagent |
HOXA9 qPCR R |
This paper |
PCR primers |
GTTGGCTGCTGGGTTATTG |
| sequence-based reagent |
PBX3 qPCR F |
This paper |
PCR primers |
CCACCAGATCATGACCATCAC |
| sequence-based reagent |
PBX3 qPCR R |
This paper |
PCR primers |
AAGAGCGCTGGTTTCATTCT |
| sequence-based reagent |
CEBPA qPCR F |
This paper |
PCR primers |
CCTTCAACGACGAGTTCCT |
| sequence-based reagent |
CEBPA qPCR R |
This paper |
PCR primers |
GCCCGGGTAGTCAAAGTC |
| sequence-based reagent |
CSF1R qPCR F |
This paper |
PCR primers |
GCCATCCACCTCTATGTCAAA |
| sequence-based reagent |
CSF1R qPCR R |
This paper |
PCR primers |
AGCAGACAGGGCAGTAGT |
| sequence-based reagent |
B2M qPCR F |
This paper |
PCR primers |
CTCTCTCTTTCTGGCCTGGAG |
| sequence-based reagent |
B2M qPCR R |
This paper |
PCR primers |
TCTGCTGGATGACGTGAGTA |
| sequence-based reagent |
SPI1 qPCR F |
This paper |
PCR primers |
TGCCCTATGACACGGATCTA |
| sequence-based reagent |
SPI1 qPCR R |
This paper |
PCR primers |
GTCCCAGTAATGGTCGCTATG |
| sequence-based reagent |
CSF3R qPCR F |
This paper |
PCR primers |
CTATGGCAAGGCTGGGAAA |
| sequence-based reagent |
CSF3R qPCR R |
This paper |
PCR primers |
GGGCTGAGACACTGATGTG |
| sequence-based reagent |
PIM1 qPCR F |
This paper |
PCR primers |
GTGGAGAAGGACCGGATTTC |
| sequence-based reagent |
PIM1 qPCR R |
This paper |
PCR primers |
TTCTTCAGCAGGACCACTTC |
| sequence-based reagent |
PBX3 qPCR F |
This paper |
PCR primers |
CAAAGAAACATGCCCTGAACTG |
| sequence-based reagent |
PBX3 qPCR R |
This paper |
PCR primers |
CTCTGATGCTGAGACCTGTTT |
| sequence-based reagent |
18S (RNA18S5) F |
This paper |
PCR primers |
CGCAGCTAGGAATAATGGAATAGG |
| sequence-based reagent |
18S (RNA18S5) R |
This paper |
PCR primers |
GCCTCAGTTCCGAAAACCAA |
| sequence-based reagent |
CIITA qPCR F |
This paper |
PCR primers |
CTGTGCCTCTACCACTTCTATG |
| sequence-based reagent |
CIITA qPCR R |
This paper |
PCR primers |
GTCGCAGTTGATGGTGTCT |
| sequence-based reagent |
EZH2 qPCR F |
This paper |
PCR primers |
GGAGGATCACCGAGATGATAAAG |
| sequence-based reagent |
EZH2 qPCR R |
This paper |
PCR primers |
TTCTGCTGTGCCCTTATCTG |
| sequence-based reagent |
EED qPCR F |
This paper |
PCR primers |
CTGGCACAGTAAAGAAGGAGAT |
| sequence-based reagent |
EED qPCR R |
This paper |
PCR primers |
GCATCAGCATCCACGTAAGA |
| sequence-based reagent |
MEF2C promoter qPCR F |
This paper |
PCR primers |
TCTGGACGAGTCTGGTTACTT |
| sequence-based reagent |
MEF2C promoter qPCR R |
This paper |
PCR primers |
AGGAAGAAGGAGGAGGAAGAG |
| sequence-based reagent |
PIM1 promoter qPCR F |
This paper |
PCR primers |
ctcagcgaaacggagagc |
| sequence-based reagent |
PIM1 promoter qPCR R |
This paper |
PCR primers |
cgtatcgattcaaacccaaacaa |
| sequence-based reagent |
FLT3 promoter qPCR F |
This paper |
PCR primers |
ctttctcagggcctcaaagat |
| sequence-based reagent |
FLT3 promoter qPCR R |
This paper |
PCR primers |
ccgaactctgtcgtttggat |
| sequence-based reagent |
ARID3B promoter qPCR F |
This paper |
PCR primers |
acgagaacctctgaggaaga |
| sequence-based reagent |
ARID3B promoter qPCR R |
This paper |
PCR primers |
gctgggaggaaagtaactaaaga |
| sequence-based reagent |
CSF3R promoter qPCR F |
This paper |
PCR primers |
GCAGAACCATTGTGGGTAAAC |
| sequence-based reagent |
CSF3R promoter qPCR R |
This paper |
PCR primers |
ggcagatggagaaacaggaa |
| sequence-based reagent |
BCL6 promoter qPCR F |
This paper |
PCR primers |
agctcgatctgctgagtttatg |
| sequence-based reagent |
BCL6 promoter qPCR R |
This paper |
PCR primers |
gcctctggaattctgagaactaat |
| sequence-based reagent |
HOXA9 promoter qPCR F |
This paper |
PCR primers |
GCCTTATGGCATTAAACCTGAAC |
| sequence-based reagent |
HOXA9 promoter qPCR R |
This paper |
PCR primers |
GAGGAGAACCACAAGCATAGTC |
| sequence-based reagent |
MEIS1 promoter qPCR F |
This paper |
PCR primers |
GGAGAGAGAGGGAGAGAAAGAA |
| sequence-based reagent |
MEIS1 promoter qPCR R |
This paper |
PCR primers |
CAAATGCACAAAGCCCTAGC |
| sequence-based reagent |
HLA-DRA promoter qPCR F |
This paper |
PCR primers |
CAGAGCGCCCAAGAAGAA |
| sequence-based reagent |
HLA-DRA promoter qPCR R |
This paper |
PCR primers |
cctcagcacctacCTTTGATAG |
| sequence-based reagent |
intergenic qPCR F |
This paper |
PCR primers |
TACACGACAGAGGACTGGAA |
| sequence-based reagent |
intergenic qPCR R |
This paper |
PCR primers |
CCTTCATGGGTGAGGGTAATG |
| sequence-based reagent |
scrambled shRNA F |
Yuan et al., 2009 |
shRNA construct for gene knockdown |
CCGG TTCTCCGAACGTGTCACGTTT CTCGAG AAACGTGACACGTTCGGAGAA TTTTT |
| sequence-based reagent |
scrambled shRNA R |
Yuan et al., 2009 |
shRNA construct for gene knockdown |
AATTAAAAA TTCTCCGAACGTGTCACGTTT CTCGAG AAACGTGACACGTTCGGAGAA |
| sequence-based reagent |
FLT3 shRNA F |
Green et al., 2015 |
shRNA construct for gene knockdown |
CCGG GCATCCCAGTCAATCAGCTTT CTCGAG AAAGCTGATTGACTGGGATGC TTTTT |
| sequence-based reagent |
FLT3 shRNA R |
Green et al., 2015 |
shRNA construct for gene knockdown |
AATTAAAAA GCATCCCAGTCAATCAGCTTT CTCGAG AAAGCTGATTGACTGGGATGC |
| sequence-based reagent |
GFP shRNA F |
Scheeren et al., 2005 |
shRNA construct for gene knockdown |
CCGG GCAAGCTGACCCTGAAGTTCAT CTCGAG ATGAACTTCAGGGTCAGCTTGC TTTTT |
| sequence-based reagent |
GFP shRNA R |
Scheeren et al., 2005 |
shRNA construct for gene knockdown |
AATTAAAAA GCAAGCTGACCCTGAAGTTCAT CTCGAG ATGAACTTCAGGGTCAGCTTGC |
