Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Bordetella pertussis) | Fluc-Bpe | NCBI | WP_003818609.1 | Bears mutation R28K to increase yield (PMID:26344196) |
| Gene (Escherichia coli virulence plasmid) | Fluc-Ec2 | NCBI | WP_001318207.1 | Bears mutation R25K to increase yield (PMID:26344196). For cysteine modification experiments, C74A (this paper — see Figure 1—figure supplement 1). |
| Recombinant DNA reagent | Fluc-Bpe in pET21a (plasmid) | PMID:26344196 | Expression vector for Fluc-Bpe. Available upon request. | |
| Recombinant DNA reagent | Fluc-Ec2 in pET21a (plasmid) | PMID:26344196 | Expression vector for Fluc-Ec2. Available upon request. | |
| Chemical compound, drug | Isethionic acid | Wako Chemicals, Richmond VA | 107-36-8 | |
| Chemical compound, drug | MTSES | Toronto Research Chemicals | S672000 | |
| Chemical compound, drug | E. coli polar lipids | Avanti, Alabaster, AL | #100600C | |
| Chemical compound, drug | n-decyl-β -D-maltopyranoside | Anatrace, Maumee, OH | D322 | |
| Other | Monobodies S9 and S12 | PMID:25290819 | Purified from E. coli according to the protocol described in the reference. PMID:25290819 |