Table 5. Liposome efflux experiments: compiled results from all replicates.
| Construct | Anion | Figure | Rate (ions/s): Prep 1 | Rate (ions/s): Prep 2 | Mean ± SEM |
|---|---|---|---|---|---|
| Ec2 WT | F- | 1-S1 | >104, >104 | >104, >104 | >104 |
| Ec2 C74A | F- | 1-S1 | >104, >104 | >104, >104 | >104 |
| Ec2 WT | F- | 2a | >104, >104 | >104, >104 | >104 |
| Ec2 S81A | F- | 2a | >104, >104, >104 | >104, >104, >104 | >104 |
| Ec2 S81T | F- | 2a | <100, <100 | <100, <100 | <100 |
| Ec2 S81C | F- | 2a | <100, <100 | <100, <100 | <100 |
| Ec2 S81A/S82A | F- | 2a | 8860, 6400 | 9640, 7840, 8860 | 8320 ± 560 ions/sec |
| Ec2 S81A/S82A | Cl- | 2-S2 | <50, <50 | <50, <50 | <50 |
| Bpe S83A/T84A | Cl- | 2-S2 | <50, <50 | <50, <50 | <50 |
| Bpe T39V | F- | 3-S1 | <100, <100 | <100, <100 | <100 |
| Bpe T39S | F- | 3-S1 | >104, >104, >104 | >104, >104 | >104 |
| Bpe T39C | F- | 3-S1 | <100, <100, <100 | <100, <100 | <100 |
| Bpe T39A | F- | 3-S1 | <100, <100 | <100, <100 | <100 |
| Bpe T39N | F- | 3-S1 | <100 | <100, <100 | <100 |
| Bpe E88A | F- | 3-S1 | >104, >104 | >104, >104, >104 | >104 |
| Bpe E88Q | F- | 3-S1 | >104, >104 | >104, >104, >104 | >104 |
| Bpe E88D | F- | 3-S1 | >104, >104 | >104, >104, >104 | >104 |
| Bpe E88K | F- | 3-S1 | <100 | <100, <100 | <100 |
| Bpe Y104F | F- | 3-S1 | >104, >104, >104 | >104, >104 | >104 |
| Bpe Y104S | F- | 3-S1 | <100, <100, <100 | <100, <100 | <100 |
| Bpe Y104H | F- | 3-S1 | <100, <100 | <100, <100 | <100 |
| Bpe Y104I | F- | 3-S1 | <100, <100 | <100 | <100 |
| Bpe I50W | F- | 1-S1 | 600, 720, 960 | 650, 550, 720 | 700 ± 60 ions/sec |
| Bpe Y104F | Cl- | 4-S1 | <50, <50 | <50, <50 | <50 |
| Bpe T39S | Cl- | 4-S1 | <50, <50 | <50, <50 | <50 |
| Bpe E88Q | Cl- | 4-S1 | <50, <50 | <50, <50 | <50 |
| Bpe E88A | Cl- | 4-S1 | <50, <50 | <50, <50 | <50 |