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Advantages of PCR
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| Precise identification of bacterial strains with divergent phenotype |
| The ease of quantification |
| The ability to quantify multiple targets in the clinical specimen |
| Quality control |
| Precision |
| One-minute method for rapid analysis with larger sample size |
| Rapid analysis |
| The ability to search for various organisms or genes in one reaction |
| Least contamination |
| The study of strictly anaerobic infections |
| Greater sensitivity |
| Detection of viruses and mRNA expression levels |
| Various million times amplification of DNA or RNA |
| Ability to detect very small amounts of samples |
| Reproducibility |
| Facilitating the detection of bacterial DNA present at very low levels |
| Bacterial identification from bacterial colonies |
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Limitations of PCR
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| The enormous cost (high test and equipment cost) |
| Need to achieve high technical skills |
| Altering the specificity of amplified PCR product |
| False-positive/False-negative results |
| There are limitations to creating a high sterile environment |
| DNA contamination |
| Low ability to detect between closely related and also highly recombinant species |
| There are limitations in multiplex PCR mixing different primers |
| Capable of contaminating other reaction vials |
