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The scheme of the ACB test. Serum samples (100 μL) are added in the wells of the microplate (a), then add 25 μL of CoCl2 (b), incubate for 10 min, and then add dithiothreitol (c), which binds to free cobalt, staining the medium brown. The color intensity is proportional to the amount of free cobalt and the amount of IMA.
