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. Author manuscript; available in PMC: 2021 Jul 27.
Published in final edited form as: Cell Rep. 2021 Jul 13;36(2):109357. doi: 10.1016/j.celrep.2021.109357

Figure 2. BRG1 regulates activity-induced E-P looping and enhancer activities.

Figure 2.

(A) ChIP-qPCR assay of RNA Pol II binding to the promoters and enhancers of ARGs in wild-type and Brg1cko neurons after KCl stimulation. The CD4 gene was shown as a negative control.

(B) ChIP-qPCR analyses of H3K27Ac at c-Fos e2 and Arc enhancer (e) in wild-type and Brg1cko neurons after KCl stimulation.

(C) qRT-PCR analyses of eRNA levels of c-Fos e2 and Arc e in Brg1cko and control neurons after KCl treatment.

(D and E) E-P crosslinking frequency in 3C experiments of c-Fos (D) and Arc (E) in basal or depolarized wild-type and Brg1cko neurons. The genomic structures and the PCR primer locations (triangles) are shown above the results. The anchor primer was paired with other primers to measure the interactions.

(F) ChIP-qPCR analyses of STAG2 levels at enhancers and promoters of c-Fos and Arc in wild-type and Brg1cko neurons after KCl stimulation.

Student’s t test (n = 3), *p < 0.05, **p < 0.01. See also Figure S3.