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. Author manuscript; available in PMC: 2021 Jul 28.
Published in final edited form as: Nat Struct Mol Biol. 2021 Feb 25;28(3):290–299. doi: 10.1038/s41594-021-00564-y

Extended Data Fig. 7 ∣. Functional validation of the C-terminal tail (CTT) of STRIP1.

Extended Data Fig. 7 ∣

a, Effects of mutation in STRIP1 on STRIPAK formation. Control or STRIP1 KO 293A cells were transfected with mock vector, FLAG-STRIP1 WT or ΔCTT mutant plasmid as indicated. Lysates and FLAG IPs were subjected to immunoblotting. b, Immunoblot of lysates of control or STRIP1 KO 293A cells transfected with mock vector, STRIP1 WT or ΔCTT mutant plasmid as indicated. HM indicates hydrophobic motif in LATS1/2. c, Quantification of the ratios of phospho- and total proteins in c. d, Immunofluorescence staining of YAP localization in 293A control or STRIP1 KO cells transfected with mock vector, STRIP1 WT or ΔCTT mutant plasmids. Scale bar, 10 μm.