FIG 1.

NF-κB subunits RelA and RelB are recruited to the HIV-1 LTR prior to activating or repressive epigenetic histone modifications. ChIP-qPCR detection of RelA/p65 (A) and Rel B (B) as well as H3Ac and H3K9me3 on the HIV-1 LTR promoter (C and D), the viral gag gene (E and F), and the NLuc gene inserted in place of nef (G and H). Assays were determined at the indicated times in CEM-SS cells expressing or not expressing HTLV-1 Tax. Levels were normalized to input DNA and are expressed as percentage of input. n = 3 biological replicates. Error bars show standard deviations (SD). **, P < 0.01, and ***, P < 0.001, calculated by two-way analysis of variance (ANOVA) with Tukey’s multiple-comparison test.