TABLE 1.
Differentially expressed genes in the C. difficile ΔselD strain compared to those in the wild-type strain
| Name | Fold change | Annotation or KEGG function | KEGG pathway |
|---|---|---|---|
| Upregulated | |||
| CDR20291_0963 | 70.544 | Alginate O-acetyltransferase complex protein | Unclassified metabolism |
| CDR20291_0962 | 44.982 | Putative uncharacterized protein | Unknown |
| CDR20291_0440 | 17.707 | Cell surface protein (putative hemagglutinin/adhesin) | Unknown |
| CDR20291_1747 | 12.712 | Putative conjugative transposon regulatory protein | Unknown |
| cysM | 8.129 | Putative O-acetylserine sulfhydrylase | Energy and amino acid metabolism |
| cysA | 5.460 | Serine O-acetyltransferase | Energy and amino acid metabolism |
| mtlF | 4.901 | Phosphotransfer system (PTS), mannitol-specific IIA component | Carbohydrate metabolism, transferases |
| mtlD | 4.753 | Mannitol-1-phosphate 5-dehydrogenase | Carbohydrate metabolism, oxidoreductases |
| CDR20291_1260 | 4.374 | Putative membrane protein | Unknown |
| mtlR | 3.886 | Mannitol operon transcriptional antiterminator | Transcription factors |
| CDR20291_2627 | 3.031 | Cytosine permease | Transporters |
| mtlA | 2.984 | PTS system, mannitol-specific IIBC component | Carbohydrate metabolism, transferases |
| CDR20291_2626 | 2.978 | Putative carbon-nitrogen hydrolase | Unknown |
| CDR20291_0474 | 2.623 | Putative exported protein | Unknown |
| ribH | 2.430 | 6,7-Dimethyl-8-ribityllumazine synthase (riboflavin synthase beta chain) | Metabolism of cofactors and vitamins, transferases |
| CDR20291_1593 | 2.319 | Putative arsenical pump membrane protein | Unknown |
| ribD | 2.232 | Diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino) uracil reductase (riboflavin biosynthesis protein) | Metabolism of cofactors and vitamins, cell community, oxidoreductases, hydrolases |
| fdhA | 2.185 | l-Seryl-tRNA (Ser) seleniumtransferase | Metabolism of other amino acids, translation, transferases |
| modA | 2.176 | Molybdate transport system substrate-binding protein | Membrane transport, transporters |
| CDR20291_0182 | 2.171 | Putative membrane-associated metalloprotease | Unknown |
| CDR20291_2397 | 2.167 | TetR-family transcriptional regulator | Unknown |
| CDR20291_0146 | 2.153 | Putative riboflavin transporter | Unknown |
| CDR20291_0178 | 2.152 | Cyclopropane-fatty-acyl-phospholipid synthase | Unclassified metabolism, transferases |
| CDR20291_2191 | 2.145 | Putative pilin protein, general secretion pathway protein G | Membrane transport, secretion system |
| CDR20291_1446 | 2.124 | Prophage antirepressor-related protein | Unknown |
| CDR20291_2012 | 2.100 | ABC-2 type transport system ATP-binding protein | Transporters |
| CDR20291_1405 | 2.089 | Putative polysaccharide deacetylase | Unknown |
| ribB | 2.083 | Riboflavin synthase | Metabolism of cofactors and vitamins, transferases |
| CDR20291_0763 | 2.073 | Aconitate hydratase | Carbohydrate metabolism, lyases |
| glyA | 2.069 | Glycine hydroxymethyltransferase | Carbohydrate, energy, amino acid, other amino acid, and cofactors and vitamin metabolism, transferases |
| iorB | 2.027 | Indolepyruvate ferredoxin oxidoreductase, beta subunit | Unclassified metabolism, oxidoreductases |
| CDR20291_1816 | 2.007 | Putative lipoprotein | Unknown |
| CDR20291_0765 | 2.001 | MarR family transcriptional regulator | Unknown |
| Downregulated | |||
| nfo | 2.042 | Deoxyribonuclease IV | Replication and repair, hydrolases |
| CDR20291_1819 | 2.090 | Putative phage-related deoxycytidylate deaminase (putative late competence protein) | Nucleotide metabolism, secretion systems, hydrolases |
| CDR20291_0177 | 2.112 | Putative oxidoreductase, NAD/FAD binding subunit | Unknown |
| CDR20291_3153 | 2.114 | Type IV pilus assembly protein | Secretion systems |
| CDR20291_1319 | 2.257 | Putative phage shock protein | Unknown |
| CDR20291_1685 | 2.274 | Putative exonuclease | Unknown |
| CDR20291_0175 | 2.333 | Anaerobic carbon-monoxide dehydrogenase catalytic subunit | Energy metabolism, xenobiotics biodegradation and metabolism, oxidoreductases |
| CDR20291_2875 | 2.424 | Conserved hypothetical protein | Unknown |
| CDR20291_2931 | 2.599 | Putative amino acid permease | Unknown |
| CDR20291_2077 | 3.005 | Putative sodium-dicarboxylate symporter | Unknown |
| CDR20291_2932 | 3.055 | Putative glutamine amidotransferase | Peptidases |
| selD | 49.582 | Selenide, water dikinase | Metabolism of other amino acids, transferases |