TABLE 3.
Conjugation frequency and fold change in conjugation frequencies for natural plasmids after ciprofloxacin exposure with or without EDAa
| Strains | Plasmid | Resistance determinant | MIC (μg/ml) | Control |
1/2 MIC |
1/2 MIC + EDA |
|||
|---|---|---|---|---|---|---|---|---|---|
| CF | Fold change | CF | Fold change | CF | Fold change | ||||
| RZ211 | pOX38 | bla CTX-M-15 | 0.004 | 1.1 × 100 ± 8.4 × 10−1 | 1 | 2.3 × 101 ± 5.8 × 100 | 19.2 | 5.4 × 100 ± 2.6 × 100 | 4.5 |
| KPM26 | IncFIB | bla KPC-3 | 1 | 4 × 10−6 ± 5.5 × 10−8 | 1 | 1 × 10−4 ± 1.7 × 10−5 | 24.6 | 1.2 × 10−5 ± 1 × 10−5 | 3 |
| KPM2 | IncN | bla KPC-3 | 0.016 | 6.9 × 10−6 ± 7 × 10−6 | 1 | 5 × 10−6 ± 8 × 10−6 | 0.7 | 2.5 × 10−6 ± 3.5 × 10−6 | 0.4 |
| KPI1 | IncI1 | bla CTX-M-15 | 0.032 | 4.7 × 10−7 ± 1 × 10−7 | 1 | 4.8 × 10−6 ± 1.6 × 10−6 | 10.2 | 6.2 × 10−7 ± 2.9 × 10−7 | 1.3 |
| KP11978 | IncL/M | bla OXA-48 | 1.5 | 2.1 × 10−4 ± 5.3 × 10−5 | 1 | 9.2 × 10−4 ± 1.2 × 10−4 | 4.1 | 1.7 × 10−5 ± 7.3 × 10−6 | 0.1 |
| EC1010 | IncFII | bla CTX-M-15 | 0.032 | 5.7 × 10−5 ± 5.5 × 10−5 | 1 | 1 × 10−3 ± 5.5 × 10−4 | 17.5 | 5 × 10−4 ± 2 × 10−4 | 8.7 |
a
EDA, edaravone (0.1 mM); CF, conjugation frequency. Statistically significant changes are in bold (P < 0.05).