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. 2021 May 18;65(6):e02658-20. doi: 10.1128/AAC.02658-20

TABLE 5.

Expression of two chromosomally encoded SOS-related genes for clinical E. coli isolates harboring different types of plasmids after exposure to sub-MIC of ciprofloxacin

Gene 2−ΔΔCT for strain at indicated CIP concn (μg/ml)a
KPI1 (IncI1, blaCTX-M-15)
KPM2 (IncN, blaKPC-3)
KPM26 (IncFIB, blaKPC-3)
KP11978 (IncL/M, blaOXA-48)
EC1010 (IncFII, blaCTX-M-15)
0 0.016 0 0.008 0 0.5 0 0.75 0 0.016
recA 1 8.6 ± 0.4 1 0.7 ± 0.15 1 4.5 ± 0.9 1 4.1 ± 0.15 1 3.3 ± 0.7
sfiA 1 0.6 ± 0.1 1 0.2 ± 0.05 1 10.5 ± 0.1 1 1.1 ± 0.07 1 0.8 ± 0.25
a

Fold change in mRNA levels were determined by RT-qPCR. The data were normalized to the mRNA levels of the 16S rRNA gene. Three independent replicates were performed. Bold indicates significant results (P < 0.05).