TABLE 5.
Expression of two chromosomally encoded SOS-related genes for clinical E. coli isolates harboring different types of plasmids after exposure to sub-MIC of ciprofloxacin
| Gene | 2−ΔΔCT for strain at indicated CIP concn (μg/ml)a |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| KPI1 (IncI1, blaCTX-M-15) |
KPM2 (IncN, blaKPC-3) |
KPM26 (IncFIB, blaKPC-3) |
KP11978 (IncL/M, blaOXA-48) |
EC1010 (IncFII, blaCTX-M-15) |
||||||
| 0 | 0.016 | 0 | 0.008 | 0 | 0.5 | 0 | 0.75 | 0 | 0.016 | |
| recA | 1 | 8.6 ± 0.4 | 1 | 0.7 ± 0.15 | 1 | 4.5 ± 0.9 | 1 | 4.1 ± 0.15 | 1 | 3.3 ± 0.7 |
| sfiA | 1 | 0.6 ± 0.1 | 1 | 0.2 ± 0.05 | 1 | 10.5 ± 0.1 | 1 | 1.1 ± 0.07 | 1 | 0.8 ± 0.25 |
a
Fold change in mRNA levels were determined by RT-qPCR. The data were normalized to the mRNA levels of the 16S rRNA gene. Three independent replicates were performed. Bold indicates significant results (P < 0.05).