TABLE 1.
Summary of viral and antibody titers for reinfection experimentsa
| Animal no. by rechallenge time point | Primary infection |
Secondary infection |
||||||
|---|---|---|---|---|---|---|---|---|
| Peak viral titers |
Neutralizing antibody titere |
Peak viral titers |
Neutralizing antibody titer | |||||
| Infectious virus in nasal wash (log10 TCID50/ml [day p.i.]) | vRNA copies/ml in nasal wash (day p.i.) | Day 21 | Day 28 | Day 56 | Infectious virus in nasal wash (log10 TCID50/ml [day postreinfection]) | vRNA copies/ml in nasal wash (day postreinfection) | Day 14 postreinfectionb | |
| Day 28c | ||||||||
| F2846 | 2.95 (7) | 7.26E + 06 (5) | 13 | 14 | n/a | n.d. | 1.49E + 03 (1) | 28 |
| F0584 | 1.0 (3) | 7.99E + 05 (5) | n.d. | n.d. | n/a | n.d. | 5.21E + 04 (1) | 28 |
| F2850 | 1.0 (3) | 9.72E + 04 (5) | n.d. | n.d. | n/a | n.d. | 9.82E + 03 (1) | n.d. |
| F0591 | 1.7 (3) | 3.11E + 06 (1) | 10 | n.d. | n/a | n.d. | 1.60E + 04 (1) | 25 |
| Day 56d | ||||||||
| F2848 | 2.45 (3 and 5) | 2.65E + 08 (5) | n.d. | n.d. | n.d. | n.d. | 8.09E + 03 (1) | 16 |
| F0587 | 2.7 (3) | 3.56E + 08 (5) | 8 | 25 | 14 | n.d. | 4.36E + 03 (1) | 80 |
| F0132 | 3.45 (3) | 4.66E + 08 (3) | 10 | 25 | 25 | n.d. | 2.15E + 03 (1) | 101 |
| F0581 | n.d. | 9.99E + 05 (5) | n.d. | n.d. | n.d. | n.d. | 6.98E + 04 (3) | 20 |
a
p.i., postinfection; n/a, not applicable; n.d., none detected with the following limits of detection: log10 TCID50 = 0.5, vRNA = 10, and neutralizing antibody titer = 5.
b
Day 14 postreinfection for animals challenged on day 28 and 56 corresponds to days 42 and 70, postprimary infection, respectively.
c
Animals rechallenged on day 28 were the donor animals for the respiratory droplet transmission study shown in Fig. 1G.
d
Animals rechallenged on day 56 were the donor animals for the direct contact transmission study shown in Fig. 1A.
e
Results are expressed as the reciprocal serum dilution that neutralized 100 TCID50s of SARS-CoV-2.