TABLE 3.
Thin-layer agar results versus indirect-DST and composite reference standard for resistance to ofloxacin
| OFX result by CRSa | No. of isolates | gyrA/B target (Sanger sequencing/WGS) | LPA target | Result of: |
TLA final interpretation | |
|---|---|---|---|---|---|---|
| LJ (2.0 μg/ml OFX) | TLA (2.0 μg/ml OFX) | |||||
| OFX-R (n = 1) | 1 | na/D94N | na | R | R | True OFX-R |
| OFX-S (n = 184) | 117 | na/WT | na | S | S | True OFX-S |
| 1 | na/WT | na | S | R | False OFX-R | |
| 62 | na/na | na | S | S | True OFX-S | |
| 1 | WT/na | WT | S | S | True OFX-S | |
| 3 | WT/WTb | WT | R | S | True OFX-S | |
a
CRS, composite reference standard; TLA, thin-layer agar; OFX, ofloxacin; WGS, whole-genome sequencing; na, not available.
b
For 1 isolate, WGS was not done.