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. 2021 Jun 11;87(13):e00423-21. doi: 10.1128/AEM.00423-21

FIG 4.

FIG 4

Characterization of T4 phage mutants capable of growing on cells expressing OmpCK12(P177V) or OmpCK12(F182A). (A) The suspensions containing the number of wild-type (WT) or mutant (M1) T4 phages indicated on the left were applied as spots onto an LB plate containing 0.2% l-Ara seeded with TY0807, TY0807 ΔompC, and TY0807 ΔompC harboring pBAD33-OmpCK12 or pBAD33-OmpCK12(P177V), as indicated on the bottom, and the plates were incubated overnight at 37°C. (B) The suspensions containing the number of WT or mutant (M2) T4 phages indicated on the left were applied as spots onto an LB plate containing 0.2% l-Ara seeded with TY0807, TY0807 ΔompC, and TY0807 ΔompC harboring pBAD33-OmpCK12 or pBAD33-OmpCK12(F182A), as indicated on the bottom, and the plates were incubated overnight at 37°C. (C) Adsorption analyses of T4 phage mutant (N937S) were performed as described in Materials and Methods. Symbols: ●, TY0807 ΔompC harboring pBAD33; ■, TY0807 ΔompC harboring pBAD33-OmpCK12; ▴, TY0807 ΔompC harboring pBAD33-OmpCK12(P177V). The number of unadsorbed phages was determined by plating with TY0807 as an indicator. (D) Adsorption analyses of T4 phage mutant (G942R) were performed as described in Materials and Methods. Symbols: ●, TY0807 ΔompC harboring pBAD33; ■, TY0807 ΔompC harboring pBAD33-OmpCK12; ▴, TY0807 ΔompC harboring pBAD33-OmpCK12(F182A). The number of unadsorbed phages was determined by plating with TY0807 as an indicator. (E) The DT region of T4 phage long tail fiber (gp37) viewed from the side (left) and the top (right) is shown (PDB ID 2XGF). Note that the top view is enlarged. N937 (red) and G942 (blue) are highlighted.