FIG 6.

Isolation and characterization of the T4 phage mutants capable of growing on ΔompC cells. (A) The phage library (2 × 10¹⁰ PFU) prepared with regular PCR (left plate) or error-prone PCR (right plate) was plated onto an LB plate seeded with TY0807 ΔompC cells and incubated overnight at 37°C. (B) The suspensions containing the different numbers (10¹, 10², 10³, and 10⁴) of wild-type (WT) or mutant (M1 and M2) T4 phages indicated on the top were applied as spots onto an LB plate seeded with BB, TY0807, TY0807 ΔompC, TY0726, or TY0728, and the plates were incubated overnight at 37°C. TY0807 or BB was used as a wild-type Escherichia coli K-12 strain or B strain, respectively. (C) Top views of the DT region of gp37 showing the surface (PDB ID 2XGF). S952 (blue) and Y953 (red) are highlighted.