FIG 9.

Differentiation and function of transferred NK cell subsets in recipient mice. (A) Percentages of NK subsets in recipient mice receiving DP-, SP-, or DN-NK. (B) DP-NK from different recipients (DP-DP, DP isolated from mice receiving DP-NK; SP-DP, DP isolated from mice receiving SP-NK; DN-DP, DP isolated from mice receiving DN-NK; PBS-DP, DP isolated from naive C57BL/6N mice) were purified by flow sorting and cocultured with PR8-treated bone marrow-derived DCs (target [T]) at an E/T ratio of 10:1 for 4 h at 37°C and 5% CO₂. Expressions of CD107a and IFN-γ in NK were determined by surface and intracellular staining and analyzed by flow cytometry. Anti-CD107a monoclonal antibody was added to the system at the start of coculture, while 10 mg/ml BFA was added to intracellular staining at the start of coculture to block the secretion of cytokines; n = 5. Data are shown as means ± SEMs and represent 2 independent experiments.