TABLE 2.
Growth inhibition of a panel of bacterial strains and eukaryotic cells by G9066, G2824, globomycin, and vancomycin
| Bacterium or effect | Strain and treatment | MIC or EC50 (μM)a |
|||
|---|---|---|---|---|---|
| G9066 | G2824 | Globomycin | Vancomycin | ||
| E. coli | MG1655 | >100 | 93.8 | 25 | >100 |
| MG1655 Δlpp | 31.3 | 62.5 | 50 | >100 | |
| MG1655 + EDTA | 3.1 | 3.1 | 0.8 | 3.1 | |
| MG1655 Δlpp + EDTA | 3.1 | 3.1 | 12.5 | 0.8 | |
| CFT073 | 100 | 92 | 29.2 | >100 | |
| CFT073 Δlpp | 31.3 | 62.5 | >100 | >100 | |
| CFT073 + EDTA | 3.1 | 3.1 | 1.1 | 0.8 | |
| CFT073 Δlpp + EDTA | 1.6 | 2.3 | 4.7 | 0.8 | |
| CFT073 imp4213 | 5.7 | 8.4 | 0.5 | 0.6 | |
| CFT073 imp4213 Δlpp | 6.8 | 8.9 | 11.5 | 0.7 | |
| A. baumannii | 19606 | 37.5 | 37.5 | 25 | >100 |
| 19606 + EDTA | 3.1 | 6.3 | 0.6 | 0.2 | |
| P. aeruginosa | PA14 | >100 | >100 | >100 | >100 |
| PA14 imp4213 | 6.3 | 6.3 | 50 | 12.5 | |
| PA14 + EDTA | 6.3 | 6.3 | 50 | 6.3 | |
| S. aureus | USA300 | 6.3 | >100 | >100 | 0.4 |
| Mammalian cytotoxicityb | HepG2 | >100 | >100 | >100 | >100 |
| Hela | >100 | >100 | >100 | >100 | |
| 293T | >100 | >100 | >100 | >100 | |
a
All E. coli MIC values represent averages from at least four independent experiments, each performed in duplicates. For other bacterial strains, MIC values represent averages from two independent experiments each performed in duplicates.
b
Mammalian cytotoxicity (EC50) values are representative of three independent replicates.