TABLE 2.

Results of the real-time PCR assay (expressed as melting temperatures) and the enzyme immunoassay (expressed as the reciprocal titers) among 37 patients with Bartonella endocarditis

Patient no.	Age (yrs)/sexa	Molecular diagnosisb	Conventional PCR target gene(s)	Real-time PCR Tm (°C)c			Maximal reciprocal titer (anti-Bartonella IgG antibodies)
				Tm-1	Tm-2	Tm-3	B. henselae	B. quintana	B. koehlerae
1	60/M	B. koehlerae	gltA, ribC	59	400	800	1,600
2	63/M	B. henselae	gltA	49	59	1,600	400	800
3	71/M	B. henselae	gltA	49	59	3,200	1,600	3,200
4	37/M	B. henselae	gltA	49	59	3,200	1,600	1,600
5	58/M	B. henselae	ribC, pap31	NAd	NA	NA	12,800	6,400	NDe
6	63/M	B. henselae	gltA	49	60	400	NA	ND
7	22/F	B. henselae	gltA	49	59	3,200	1,600	ND
8	71/M	B. henselae	pap31	NA	NA	NA	3,200	3,200	ND
9	74/F	B. henselae	ribC	49	59	3,200	800	ND
10	75/F	B. henselae	ribC	49	59	12,800	12,800	ND
11	73/M	B. henselae	ribC	49	59	1,600	800	ND
12	43/M	B. henselae	pap31, 16S rRNA	51	61	NA	NA	ND
13	56/M	B. henselae	gltA	49	60	1,600	ND	ND
14	71/F	B. henselae	gltA, 16S rRNA	49	59	6,400	6,400	ND
15	20/M	B. henselae	ribC	51	60	6,400	3,200	ND
16	56/F	B. henselae	16S rRNA	49	59	12,800	12,800	ND
17	50/M	B. henselae	gltA	49	59	3,200	1,600	ND
18	42/F	B. henselae	16S rRNA	50	60	12,800	6,400	ND
19	56/M	B. henselae	gltA	49	59	1,600	800	ND
20	32/M	B. quintana	gltA, groEL, rpoB, ftsZ	59	70	400	1,600	400
21	20/M	B. quintana	gltA, groEL, rpoB, ftsZ	59	70	400	1,600	200
22	56/M	B. quintana	gltA, groEL, rpoB, ftsZ	59	69	1,600	6,400	800
23	43/M	B. quintana	16S rRNA, gltA, pap31	NA	NA	NA	800	1,600	ND
24	72/M	B. quintana	gltA	NA	NA	NA	3,200	3,200	1,600
25	56/M	B. quintana	ribC	59	70	200	1,600	ND
26	48/F	B. quintana	gltA, groEL, rpoB, ftsZ	59	70	1,600	6,400	ND
27	12/F	B. quintana	gltA, groEL, rpoB, ftsZ	58	69	100	1,600	ND
28	7/F	B. quintana	gltA, groEL, rpoB, ftsZ	61	71	1,600	NA	ND
29	28/M	B. quintana	gltA, groEL, rpoB, ftsZ	61	71	3,200	6,400	ND
30	52/M	B. quintana	16S rRNA	58	69	12,800	25,600	ND
31	16/F	B. quintana	gltA, groEL, rpoB, ftsZ	58	68	12,800	6,400	ND
32	9/F	B. quintana	gltA, groEL, rpoB, ftsZ	59	70	3,200	6,400	ND
33	75/M	B. quintana	gltA, groEL, rpoB, ftsZ	58	69	25,600	25,600	ND
34	65/M	B. quintana	gltA, groEL, rpoB, ftsZ	57	68	3,200	6,400	ND
35	20/F	B. quintana	ribC	59	70	200	200	ND
36	11/M	B. quintana	gltA, groEL, rpoB, ftsZ	59	70	12,800	25,600	ND
37	75/F	B. quintana	16S rRNA	58	69	3,200	6,400	ND

^aM, male; F, female.

^bMolecular diagnosis was determined by sequencing of the conventional PCR products and by real-time PCR melting-curve analysis. All B. henselae strains were Huston-1 type as determined by DNA sequencing.

^cT_m-1, melting temperature generated by the B. henselae-specific probe; T_m-2, melting temperature generated by the Bartonella genus probe; T_m-3, melting temperature generated by the B. quintana-specific probe.

^dNA, not available. Specimen was not available for further testing.

^eND, not done.