TABLE 3.
Sequences of oligonucleotides relevant to this study
| Oligonucleotide use and name | Oligonucleotide sequence (5′→3′) | Target organism | Target gene | Product size (bp) | Reference |
|---|---|---|---|---|---|
| Conventional PCR | |||||
| BhCS.781p | GGGGACCAGCTCATGGTGG | Bartonella sp. | gltA | 379 | 37 |
| BhCS.1137 | AATGCAAAAAGAACAGTAAACA | Bartonella sp. | |||
| gltA-F | GCTATGTCTGCATTCTATCA | Bartonella sp. | gltA | 790 | 38 |
| gltA-R | GATCYTCAATCATTTCTTTCCA | Bartonella sp. | |||
| Barton-1 | TAACCGATATTGGTTGTGTTGAAG | Bartonella sp. | ribC | 588 | 39 |
| Barton-2 | TAAAGCTAGAAAGTCTGGCAACATAACG | Bartonella sp. | |||
| PAPn1 | TTCTAGGAGTTGAAACCGAT | B. henselae | pap31 | 277 | 40 |
| PAPn2 | GAAACACCACCAGCAACATA | B. henselae | |||
| ftsZ-F | ATTAATCTGCAYCGGCCAGA | Bartonella sp. | ftsZ | 935 | 38 |
| ftsZ-R | ACVGADACACGAATAACACC | Bartonella sp. | |||
| groEL-F | GAACTNGAAGATAAGTTNGAA | Bartonella sp. | groEL | 1,459 | 38 |
| groEL-R | AATCCATTCCGCCCATTC | Bartonella sp. | |||
| rpoB-F | CGCATTGGCTTACTTCGTATG | Bartonella sp. | rpoB | 893 | 38 |
| rpoB-R | GTAGACTGATTAGAACGCTG | Bartonella sp. | |||
| 16S-27f | AGAGTTTGATCMTGGCTCAG | Eubacteria | 16S rRNA | 862-3 | 41 |
| 16S-907r | CCGTCAATTCMTTTRAGTTT | Eubacteria | 42 | ||
| Real-time PCR | |||||
| ribC-bart-F1 | GGTTTGGCTGAGATYATTGA | Bartonella sp. | ribC | 185 | Present study |
| ribC-bart-R1 | TCAAGTGTATGRCGAATAAT | Bartonella sp. | Present study | ||
| Bartonella genus SimpleProbe | GAAGGTGAXITGCAGTTCGTT-Ph | Bartonella sp. | ribC | Present study | |
| B. henselae SimpleProbe | TTTCCTXIACAAGTACCAAA-Ph | B. henselae | ribC | Present study | |
| B. quintana SimpleProbe | TCCTGCAAGTXITCCAACAAAATTCACGCCCTT-Ph | B. quintana | ribC | Present study | |
| β-globin-F | TACACATATTGACCAAATCAGGGTA | Human | β-globin | 165 | Present study |
| β-globin-R | TTTAGAATGGTGCAAAGAGGC | Human | β-globin | Present study | |
| β-globin-probe | Cy5-TACTTTCCCTAATCTCTTTCTTTCAGGGCAAT-BBQ | Human | β-globin | Present study |