. 2021 May 18;65(6):e00256-21. doi: 10.1128/AAC.00256-21

TABLE 2.

In vitro susceptibility results for cultured mycelial and yeast forms of 32 Talaromyces marneffei strains against eight antifungal agents

Strain type (no. of isolates) and drug	MIC/MEC (μg/ml)^a in mycelial form				MIC (μg/ml)^a in yeast form
Strain type (no. of isolates) and drug	Range	MIC₅₀/MEC₅₀	MIC₉₀/MEC₉₀	Geometric mean	Range	MIC₅₀/MEC₅₀	MIC₉₀/ME₉₀	Geometric mean
All strains (32)
Olorofim	0.0005–0.001	0.0005	0.0005	0.0005	0.00025–0.002	0.0005	0.002	0.0007
Amphotericin B	0.5–4	2	4	1.9152	0.031–1	0.125	0.475	0.1331
Itraconazole	≤0.016	0.016	0.016	0.0160	≤0.016–0.031	0.016	0.016	0.0163
Voriconazole	≤0.016–0.063	0.063	0.063	0.0453	≤0.016–0.031	0.016	0.0295	0.0174
Posaconazole	≤0.016	0.016	0.016	0.0160	≤0.016	0.016	0.016	0.0160
Caspofungin	0.5–4	1	4	1.3543	0.25–32	8	16	4.5552
5-Flucytosine	0.031–1	0.062	0.125	0.0755	0.031–2	0.25	0.95	0.2443
Terbinafine	0.125–0.25	0.125	0.25	0.1393	0.031–0.5	0.125	0.25	0.1168

Clinical (17)
Olorofim	0.0005–0.001	0.0005	0.0005	0.0005	0.00025–0.002	0.0005	0.02	0.0007
Amphotericin B	0.5–4	2	4	2	0.031–1	0.125	0.5	0.1252
Itraconazole	≤0.016	0.016	0.016	0.016	≤0.016	0.016	0.016	0.016
Voriconazole	≤0.016–0.063	0.063	0.063	0.0419	≤0.016–0.031	0.016	0.031	0.0173
Posaconazole	≤0.016	0.016	0.016	0.016	≤0.016	0.016	0.016	0.016
Caspofungin	0.5–4	2	4	1.8434	0.25–32	2	16	2.6606
5-Flucytosine	0.031–1	0.063	0.125	0.0834	0.031–2	0.25	1	0.2825
Terbinafine	0.125–0.25	0.125	0.25	0.1471	0.031–0.5	0.125	0.25	0.1252

Animal (11)
Olorofim	0.0005–0.0005	0.0005	0.0005	0.0005	0.00025–0.002	0.0005	0.002	0.0006
Amphotericin B	1–4	2	2	1.7631	0.063–0.5	0.125	0.25	0.1512
Itraconazole	≤0.016	0.016	0.016	0.016	≤0.016–0.031	0.016	0.016	0.017
Voriconazole	0.031–0.063	0.063	0.063	0.0519	≤0.016–0.031	0.016	0.031	0.018
Posaconazole	≤0.016	0.016	0.016	0.016	≤0.016	0.016	0.016	0.016
Caspofungin	0.5–4	1	2	1.065	1–16	16	16	8.5203
5-Flucytosine	0.063–0.125	0.063	0.125	0.0714	0.031–0.5	0.125	0.5	0.1714
Terbinafine	0.125–0.25	0.125	0.125	0.1331	0.031–0.25	0.063	0.25	0.0971

Environmental (4)
Olorofim	0.0005–0.0005	0.0005	0.0005–0.002	0.0008
Amphotericin B	2–2	2	0.063–0.25	0.1252
Itraconazole	≤0.016	0.016	≤0.016	0.016
Voriconazole	0.031–0.063	0.0442	≤0.016	0.016
Posaconazole	≤0.016	0.016	≤0.016	0.016
Caspofungin	0.5–1	0.7071	2–32	8
5-Flucytosine	0.031–0.125	0.0626	0.125–1	0.3536
Terbinafine	0.125–0.125	0.125	0.063–0.25	0.1489

MEC, minimal effective concentration; MIC₅₀/MEC₅₀, minimal concentration that inhibits 50% of isolates; MIC₉₀/MEC₉₀, minimal concentration that inhibits 90% of isolates. MECs were used for caspofungin.