Skip to main content
. 2021 Jun 11;87(13):e00442-21. doi: 10.1128/AEM.00442-21

FIG 2.

FIG 2

At least one 30- to 50-kDa protein in B. subtilis subsp. natto supernatant inhibits pCF10 transfer. (A) Mating culture of E. faecalis treated with different MW fractions of B. subtilis subsp. natto supernatant. In this picture, the turbidity of the culture on the left, which was treated with the fraction with a MW less than 30 kDa appears higher than that of the culture on the right, which was treated with the fraction with a MW greater than 30 kDa. (B) Effect of different MW fractions of B. subtilis subsp. natto supernatant on the conjugative transfer of pCF10. The y axis indicates the T/D ratio, and the x axis indicates different MW (kDa) fractions of B. subtilis subsp. natto supernatant. Ctrl, negative control (the group treated with fresh M9B medium; no SCS treatment); untreated, the group treated with unfractionated B. subtilis subsp. natto supernatant. Each dot in the figure represents a replication. The red diamonds with red lines indicate the means ± SDs (n = 3). Multiple comparisons were evaluated using Duncan’s multiple-range test. The different uppercase letters above the dots indicate the significance of differences between groups (P < 0.05). (C) SDS-PAGE of B. subtilis subsp. natto supernatant. Lanes 1 and 7, protein marker; lane 2, B. subtilis subsp. natto supernatant (5 μl); lane 3, B. subtilis subsp. natto supernatant (10 μl); lane 4, M9B medium (5 μl); lane 5, M9B medium (10 μl); lane 6, BSA.