TABLE 2.
Bacterial strains and plasmids used
| Strain or plasmid | Genotypes or characteristicsa | Source or referenceb |
|---|---|---|
| Strains | ||
| KP7600 | W3110 type A; F− lacIq lacZΔM15 λ− galK2 galT22 IN(rrnD-rrnE)1 | NBRP |
| HVA10 | Highly virulent mutant from KP7600 carrying opgH Trp30stop | This study |
| HVA13 | Highly virulent mutant from KP7600 carrying opgH Gly360Glu | This study |
| HVA23 | Highly virulent mutant from KP7600 carrying opgH Glu464Lys | This study |
| HVK4a | Highly virulent mutant from KP7600 carrying opgH His407Tyr | This study |
| HVK7b | Highly virulent mutant from KP7600 carrying opgG Gln239stop | This study |
| HVK7a | Highly virulent mutant from KP7600 carrying opgH Gly316Ser | This study |
| HVA17 | Highly virulent mutant from KP7600 carrying opgH Gln398stop | This study |
| HVK6a | Highly virulent mutant from KP7600 carrying opgH Trp673stop | This study |
| HVA22 | Highly virulent mutant from KP7600 carrying opgG Gln67stop | This study |
| HVK5a | Highly virulent mutant from KP7600 carrying opgH Trp806stop | This study |
| JD22094 | KP7600 opgG::mini-Tn10 (inserted 178 bp from initial codon) Kanr | NBRP |
| JD25108 | KP7600 envZ::mini-Tn10 Kanr | NBRP |
| JD25110 | KP7600 ompR::mini-Tn10 Kanr | NBRP |
| JW1035 | BW25113 opgG::kan Kanr | NBRP |
| JW2367 | BW25113 evgS::kan Kanr | NBRP |
| K0900 | KP7600 ΔopgG::markerless (transduced from Keio Collection JW1035) | This study |
| K0901 | KP7600 ΔopgH::markerless | This study |
| K1000 | KP7600 opgGH::cat Cmr | This study |
| K1001 | KP7600 rcsB::cat Cmr | This study |
| K1002 | KP7600 wza::cat Cmr | This study |
| K1003 | KP7600 evgS::kan Kanr (transduced from Keio Collection JW2367) | This study |
| K1004 | KP7600 rcsB::cat Cmr opgG::mini-Tn10 Kanr | This study |
| K1006 | KP7600 wza::cat Cmr opgG::mini-Tn10 Kanr | This study |
| K1008 | KP7600 evgS::kan Kanr opgGH::cat Cmr | This study |
| K1009 | KP7600 envZ::mini-Tn10 Kanr opgGH::cat Cmr | This study |
| K1010 | KP7600 ompR::mini-Tn10 Kanr opgGH::cat Cmr | This study |
| Plasmids | ||
| pMW118 | Low-copy-number plasmid; Ampr | Nippon Gene |
| popgG | pMW118 with opgG; Ampr | This study |
| popgH | pMW118 with opgH; Ampr | This study |
| popgGH | pMW118 with opgGH operon; Ampr | This study |
| pKD46 | λRed recombinase expression; temperature sensitive, Ampr | 42 |
| pKD3 | flp-cat-flp Cmr Ampr | 42 |
| pCP20 | FLP recombinase; Ampr | 42 |
a
Kanr, kanamycin resistance; Cmr, chloramphenicol resistance; Ampr, ampicillin resistance.
b
NBRP, National BioResource Project.