Comparison of optical density values for latest-generation IgG serological assays by region of birth. Wiener Lisado displayed a median S/CO of 6.95 (interquartile range [IQR], 6.05 to 7.38) for samples of donors born in South America, 4.89 (IQR, 3.96 to 5.65) for samples of donors born in Central America, and 2.68 (IQR, 2.28 to 3.34) for samples of donors born in Mexico. Wiener v.4.0 displayed a median S/CO of 5.78 (IQR, 5.43 to 6.72) for samples of donors born in South America, 5.20 (IQR, 4.69 to 6.04) for samples of donors born in Central America, and 3.67 (IQR, 3.02 to 4.07) for samples of donors born in Mexico. Abbott PRISM displayed a median S/CO of 8.92 (IQR, 7.64 to 9.71) for samples of donors born in South America, 7.97 (IQR, 6.71 to 8.59) for samples of donors born in Central America, and 5.09 (IQR, 4.42 to 6.60) for samples of donors born in Mexico. Bar and whiskers represent median with interquartile range. ns, P ≥ 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.