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Serum neutralization of autologous isolates and some heterologous tier 2 isolates cannot be blocked by V3 peptide. Neutralization by monoclonal antibody F425-B4e8 that binds to the V3 crown (A) and sera from the final bleed (B) was tested in a neutralization assay against HIV isolates in the presence or absence of V3 peptide (JRFL sequence, NTRLSIHIGPGRAFYTTGEIIGDI).
