FIG 6.
Human STING agonist diABZI can efficiently block SARS-CoV-2 infection. (A) Introducing ACE2 stable expression in A549 cells. Whole-cell lysates harvested from A549 parental or ACE2 stable cells were immunoblotted using the indicated antibodies. GAPDH was used as a loading control. (B) A549/ACE2 cells stably expressing STINGWT were incubated with DMSO or diABZI for 3 h before treatment with SARS-CoV-2. At 24 h postinfection, cells were immunostained with antibodies against SARS-CoV-2 N protein (SARS-CoV-2 N) and STING and counterstained with DAPI. Bar: 50 μm. (C) Whole-cell lysates of the cells treated as in (B) were immunoblotted using the indicated antibodies. GAPDH was used as a loading control.