FIG 7.

Human STING agonist diABZI can efficiently block SARS-CoV-2 infection in human lung tissue slices. Human lung tissue slices were pretreated with DMSO or 1 μM human STING agonist diABZI for 3 h in 200 μl keratinocyte serum-free medium. The slices were then treated with SARS-CoV-2 virions diluted in 1 ml keratinocyte serum-free medium at 10e6 PFU/ml. The slices were incubated at 37°C in 5% CO₂. After 10 days, slices were immunostained using an antibody against the SARS-CoV-2 N protein (SARS-CoV-2 N) and counterstained with DAPI. SARS-CoV-2 N⁺ bright red dots in the tissues represent infected cells. Bar: 50 μm.